Detailed view for EHI_122100

Basic information

TDR Targets ID: 800259
Entamoeba histolytica, acetyltransferase, putative
Source Database / ID:  AmoebaDB  

pI: 6.3279 | Length (AA): 204 | MW (Da): 21807 | Paralog Number: 8

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00132   Bacterial transferase hexapeptide (six repeats)

Gene Ontology

Mouse over links to read term descriptions.
GO:0016740   transferase activity  
GO:0016407   acetyltransferase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 190 3hjj (A) 0 187 33.00 0.0000000000064 1 1.38027 -0.71
6 189 3nz2 (A) 3 184 37.00 0.000000000013 1 1.46756 -1.23
9 193 1krr (A) 6 190 30.00 0.0000000091 1 1.33446 -0.65
70 185 4ea9 (A) 100 211 26.00 0 1 1.02423 -1.46
79 184 4n6a (A) 161 254 48.00 0.0000000002 0.99 1.06291 -0.92
171 204 4mfg (A) 124 158 29.00 0.041 0.11 0.448267 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128273)

Species Accession Gene Product
Candida albicans CaO19.7437   similar to bacterial thiogalactoside acetyltransferase (lacA), and to S. cerevisiae YJL218W
Dictyostelium discoideum DDB_G0291538   hypothetical protein
Dictyostelium discoideum DDB_G0280825   hypothetical protein
Dictyostelium discoideum DDB_G0275507   hypothetical protein
Dictyostelium discoideum DDB_G0275913   hypothetical protein
Escherichia coli b0459   maltose O-acetyltransferase
Escherichia coli b2033   putative lipopolysaccharide biosynthesis O-acetyl transferase
Escherichia coli b0342   thiogalactoside acetyltransferase
Entamoeba histolytica EHI_096770   acetyltransferase, putative
Entamoeba histolytica EHI_122100   acetyltransferase, putative
Entamoeba histolytica EHI_039180   acetyltransferase, putative
Entamoeba histolytica EHI_112790   acetyltransferase, putative
Entamoeba histolytica EHI_134640   acetyltransferase, putative
Entamoeba histolytica EHI_029610   maltose O-acetyltransferase, putative
Entamoeba histolytica EHI_143110   acetyltransferase, putative
Entamoeba histolytica EHI_157020   maltose O-acetyltransferase, putative
Entamoeba histolytica EHI_107550   bacterial transferase hexapeptide family protein
Mycobacterium leprae ML1719c   POSSIBLE TRANSFERASE
Mycobacterium tuberculosis Rv3034c   Possible transferase
Mycobacterium ulcerans MUL_1904   transferase
Saccharomyces cerevisiae YJL218W   acetyltransferase
Trichomonas vaginalis TVAG_150360   maltose O-acetyltransferase, putative
Trichomonas vaginalis TVAG_379200   maltose O-acetyltransferase, putative
Trichomonas vaginalis TVAG_444570   maltose O-acetyltransferase, putative
Trichomonas vaginalis TVAG_143500   conserved hypothetical protein

Essentiality

EHI_122100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0342 Escherichia coli non-essential goodall
b0459 Escherichia coli non-essential goodall
b2033 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_122100 (Entamoeba histolytica), acetyltransferase, putative
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